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Argireline
Research Use
Also known as Acetyl Hexapeptide-3, Acetyl Hexapeptide-8
Function
Primary
Expression lines · Forehead/crow's-feet wrinkles
Mechanism
Mimics SNAP-25 N-terminus — competitively inhibits SNARE complex formation, reducing catecholamine release and micro-contractions in mimic muscles.
Evidence
Clinical RCT (Limited)
Research
Cosmetic RCTs vs placebo
Dosing
Range
5–10% in topical formulation
Frequency
1–2× daily
Route
Topical
Administration & Technique
Topical
Expression-line areas (forehead, crow's feet).
DeviceSerum or cream (5–10%).
TechniqueApply AM and PM to expression lines. Full effect ~4 weeks.
Injection & Application Sites
Front
Back
Topical
- Face / neck (topical) — Clean dry skin, AM and PM, before moisturizer.
Diagram is a general illustration, not a medical instruction. Rotate sites every injection, keep 1 in (2.5 cm) between recent sites, and avoid scars, moles, bruises and the navel area.
Cycling
Continuous topical use.
Timing & Interactions
When to take
Topical AM and PM to expression-line areas.
Do not combine with
- ·No systemic interactions
Complements
- ·Matrixyl, GHK-Cu (offset applications)
Storage & Reconstitution
Powder (Pre-Reconstitution)
Lyophilized powder: refrigerate 2–8°C for up to 24 months, or freeze at –20°C for extended storage. Keep sealed, desiccated, and protected from light.
After Reconstitution
After reconstitution with bacteriostatic water, refrigerate at 2–8°C and use within 28 days. Discard if cloudy, discolored, or precipitated.
Notes
Do not freeze after reconstitution — freeze/thaw cycles degrade most peptides.
Reconstitution Calculator
Concentration
2.50 mg/mL
Volume per dose
0.100 mL
Insulin syringe
10.0 units (U-100)
1 mL = 100 units on a standard U-100 insulin syringe. Draw to the nearest 0.5 unit and confirm dosing with a clinician before injection.
Your Notes
Your Notes
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Benefits
- ·Reduces expression-line depth
- ·Non-invasive 'topical botox' alternative
- ·Well tolerated
Risks
- ·Mild irritation
- ·Modest effect vs injectables
Contraindications
- ·Application on broken skin
Regulatory
Cosmetic ingredient (global)
Veterinary Use (Pets)
No established veterinary protocol for Argireline. Consult a licensed veterinarian before considering off-label use in any species.
Full veterinary reference →Functional Groups
Cited Works
- Blanes-Mira C et al. A synthetic hexapeptide (Argireline) inhibits neuronal exocytosis. Int J Cosmet Sci. 2002;24:303.source